![]() In contrast, AA reduction markedly downregulated CAT1, B⁰AT, and LAT1 in the P. major of TN birds, reducing AA density resulted in upregulating the expression of all AA transporters, HSP70, GR, and AQP1, while downregulating HSP90 and AQP9. Whereas in the HS birds, inconsistent expressions were observed in the jejunum, of which CAT1, B⁰AT, and LAT1 were markedly downregulated as AA density was reduced. Reducing the AA density beyond 100% upregulated the expression of AA transporter (CAT1, B⁰AT, b0,+AT, SNAT1, LAT1), HSP70, HSP90, glucocorticoid receptor (GR), and AQP3 in the TN birds’ jejunum. There was an interaction (P<0.001) between AA density and temperature on the expression of all examined genes. Feeding 80% AAs depressed BWG, FCR, and P. The results revealed no interaction between housing temperature and AA density on growth performance or P. During 24-36 days of age, half of the birds were kept at a thermoneutral (TN) temperature of 20° C, whereas the other half were subjected to HS at 32° C for 8 h daily, making the treatment design a 4 × 2. Ross 308 chicks (n=576) were randomly assigned to 4 dietary treatments (24 reps, 6 chicks per rep), differing in AA density (110, 100, 90, and 80% of a breeder's AA specifications). Thus, we evaluated feeding different amino acid (AA) densities on live performance, Pectoralis (P.) muscles, and expression of selected AA transporters, water channels, and stress-related transcripts in a fast-growing broiler strain. Various nutritional strategies have been suggested, such as altering dietary concentrations of some nutrients. Exposure to heat stress (HS) is one of the challenges facing the broiler industry worldwide. ![]()
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